Colloquium- “Single-molecule labeling and localization microscopy” with Prof. Ying S. Hu
Colloquium
October 23, 2024
3:00 PM - 4:00 PM
Location
2SES 238
Calendar
Download iCal FileProfessor Ying S. Hu, Department of Chemistry, University of Illinois Chicago
Single-molecule localization microscopy (SMLM) breaks the optical diffraction limit by one order of magnitude and enables the study of ultra-fine subcellular structures and their functions. However, sample labeling for SMLM still employs methods developed for standard fluorescence microscopy with a much lower need for high-quality labeling at the nanoscale, particularly for fragile subcellular structures. To this end, we developed single-molecule labeling and localization microscopy, or SMLLM. SMLLM deviates from existing SMLM techniques in that the gentle labeling process preserves the structural integrity of the biological sample while achieving superresolution imaging in the labeling step. We successfully demonstrated this technique using full-length antibodies, antibody fragments, a bicyclic heptapeptide, and semiconductor quantum dots. SMLLM enabled us to achieve a quantitative molecular census and revealed massive membrane fibers essential for the immune function of dendritic cells. SMLLM paves the pathway to deploy the full potential of superresolution imaging by revealing previously unseen subcellular structures and their functional roles in health and disease.
Date posted
Oct 14, 2024
Date updated
Oct 18, 2024