Biophysics Seminar- “Gel-assisted mass spectrometry imaging” with Prof. Ruixuan Gao (UIC)
Biophysics Seminar
November 15, 2024
10:30 AM - 11:30 AM
Location
SELE 2101
Calendar
Download iCal FileRuixuan Gao
Assistant Professor, Department of Chemistry and Biological Sciences
University of Illinois Chicago
Mass spectrometry imaging (MSI) is a powerful tool for spatial investigation of native molecules in intact biological specimens. However, MSI has often been precluded from singlecell applications due to the spatial resolution limit set forth by the physical and instrumental constraints of the method. In this talk, I will present a newly developed sample preparation and imaging workflow named Gel-Assisted Mass Spectrometry Imaging (GAMSI) that overcomes the current spatial resolution limit of MSI-based spatial metabolomics. With GAMSI, we show that the spatial resolution of lipid MALDI-MSI can be enhanced to the sub-micrometer level without modifying the existing mass spectrometry hardware or analysis pipeline. This type of approach may vastly enhance the accessibility of MSI-based spatial analysis at the cellular and subcellular level.
Assistant Professor, Department of Chemistry and Biological Sciences
University of Illinois Chicago
Mass spectrometry imaging (MSI) is a powerful tool for spatial investigation of native molecules in intact biological specimens. However, MSI has often been precluded from singlecell applications due to the spatial resolution limit set forth by the physical and instrumental constraints of the method. In this talk, I will present a newly developed sample preparation and imaging workflow named Gel-Assisted Mass Spectrometry Imaging (GAMSI) that overcomes the current spatial resolution limit of MSI-based spatial metabolomics. With GAMSI, we show that the spatial resolution of lipid MALDI-MSI can be enhanced to the sub-micrometer level without modifying the existing mass spectrometry hardware or analysis pipeline. This type of approach may vastly enhance the accessibility of MSI-based spatial analysis at the cellular and subcellular level.
Date posted
Sep 25, 2024
Date updated
Nov 13, 2024