BioPhysics & Condensed Matter Seminar: Prof. Brian Cannon, Loyola University Chicago  Add To Calendar

  • Date(s): Thursday, 5/3 3:30 PM to Thursday, 5/3 4:30 PM
  • Speaker: Prof. Brian Cannon
  • Host: Prof. Ursula Perez-Salas
  • Campus Address: 2214 SES
Title: “Effect of Single-stranded Domains on the Energetics and Structural Flexibility of DNA Structure”

Single-stranded domains within structured nucleic acids disrupt local base-stacking and destabilize neighboring helical domains; however, these structural motifs also expand the conformational and functional capabilities for structured nucleic acids. Using a combination of optical melting and single-molecule FRET microscopy, we investigated the influence of variations in the number, relative distribution, and sequence of unpaired nucleotides within opposing DNA strands on their biophysical properties. A thermodynamic prediction model is described for the energetic penalty of these domains. The findings provide a framework for understanding the thermodynamic and conformational effects of these domains for the rational design of functional DNA nanostructures.

Cm-Bio 5-3 Cannon flyer