Title: Developing experiments to visualize biomolecular recognition and binding
Abstract: The function of biological systems is built on the interactions of molecular binding partners, commonly involving proteins and nucleic acids. Two molecules encounter one another diffusively, recognize their partner amongst multiple potential targets, and form site-specific contacts with association rates are sometimes at or even beyond the apparent diffusion limit. I will describe our efforts to develop new tools to visualize these processes in protein-protein interactions and DNA hybridization. We use nanosecond temperature-jump experiments to thermally dissociate bound partners and create encounter complexes present during associate, and track changes in molecular structure with time-resolved vibrational spectroscopy.
Colloq flyer 4-26 Tokmakoff