Title: Structural Events during Translocation and Proofreading in Replicative DNA Polymerase
Abstract: DNA polymerases in family B are workhorses of DNA replication that carry out the bulk of the job at a high speed with high accuracy. A polymerase in this family relies on a built-in exonuclease to correct accidental errors known as proofreading. However, it has not been observed in atomic detail how the polymerase advances one nucleotide on the DNA template strand as soon as the previous one has been replicated correctly, that is, translocation. It is even more puzzling how translocation is avoided after the primer strand is excised by the exonuclease and returned back to the polymerase active site once an error occurs. The bifurcate pathways of translocation and proofreading have been unknowingly traced out by hundreds of structures in Protein Data Bank. I will present a joint analysis of all available structures of a representative member in family B and demonstrate the orchestrated structural events during translocation and proofreading.
Cm-Bio 11-5 Ren