Wednesday Colloquium: Dr. Tolou Shokuhfar
Nanomedicine: Living Cells and Nanomaterials Interactions
Future prospects for nanotechnology and biomaterials in medical applications appear to be excellent. In orthopedic applications, there is a significant need and demand for the development of a bone implant that is bioactive and exhibits mechanical and surface properties comparable with those of natural, healthy bone. Particularly, implants with nanometer-sized surfaces have been receiving much attention recently due to their ability to mimic the dimensions of constituent components of natural bone. TiO2 nanotubes have been developed and studied as novel surface modification that promote osteointegration and may be a successful alternative to conventional implants with flat surfaces. The application of nanotechnology in single-molecule level imaging can also enable the detection of changes in living cells and proteins with super resolution. A central dogma in advanced biological research remains behind the challenges related to single-molecule level imaging in living cells and visualization of molecular machinery underlying cellular function. There is thus a demanding need for nanoscale microscopy of cells and other biological systems and materials in the liquid state, which will open up new doors for super-resolution bioimaging techniques and probably change the way we view intracellular processes. To investigate the behavior of single live cells in a high-throughput manner, in-situ scanning transmission electron microcopy (STEM) analysis techniques has been introduced. This novel technique utilizing special holders allows living cells to survive and be nourished within the STEM high vacuum environment. This platform will revolutionize the field of single cell analysis and single-molecule imaging by expanding detection and imaging limits to the nanometer spatial resolution.