Departmental Colloquium: Enrique De La Cruz
Title: "How cofilin severs an actin filament"
Abstract: The actin regulatory protein, cofilin, promotes actin assembly dynamics by severing filaments and increasing the number of ends from which subunits add and dissociate. I will present results from biochemical and biophysical solution studies that yield a thermodynamic, kinetic, structural and mechanical description of cooperative cofilactin binding and actin filament severing. The experimental data are well described by a model in which discontinuities in filament topology, and mechanics generate stress accumulation and promote fracture preferentially at junctions of bare and cofilin-decorated segments along filaments. Computational modeling and simulations indicate an intrinsic coupling between filament bending and twisting elasticity is critical for stress accumulation.
Refreshments will be served at 2:30pm in SES 2620, High Bay Conference Room